Cytotoxic activity of endophytes isolated from Allamanda blanchetii A. DC

Authors

Harsha D. Pednekar, Department of Ethanomedicine, National Institute of Traditional Medicine (Indian Council of Medical Research), KLE University, Karnataka, Indi
Harsha V. Hegde, Department of Ethanomedicine, National Institute of Traditional Medicine (Indian Council of Medical Research), KLE University, Karnataka, Indi
Pramod J. Hurakadale, Department of Microbiology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, Indi
Jyoti D. Wagawade, Department of Ethanomedicine, National Institute of Traditional Medicine (Indian Council of Medical Research), KLE University, Karnataka, Indi
Kishore G. Bhat, Department of Microbiology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, Indi
Chetana Bogar, Department of Microbiology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, Indi

Abstract

AIM:

To evaluate the cytotoxic potential of endophytic fungi isolated from

A. blanchetii

.

MATERIALS AND METHODS:

Endophytic fungi were isolated from

A. blanchetii

using surface sterilization methods, grown in different types of media to ascertain their suitability for growth. Mass culture was prepared using suitable media and extracts using several solvents were prepared and dried. These extracts were later evaluated using MTT assay for their cytotoxic potential.

RESULTS:

Hydroalcoholic extract of

Phomopsis

species exhibited better cytotoxicity (80 μg/ml) when compared ethyl acetate extract of the same 160 μg/ml. Similarly, ethyl acetate extract of

Phomopsis

species, ethyl acetate extract of

Alternaria

aff.

raphani

J. W. Groves and Skolko also demonstrated cytotoxicity of 160 μg/ml in MTT assay against MCF 7 cell line.

CONCLUSION:

Both the species of

Phomopsis

and

Alternaria

have shown significant cytotoxicity in MTT assay.

Recommended Citation

Pednekar, Harsha D.; Hegde, Harsha V.; Hurakadale, Pramod J.; Wagawade, Jyoti D.; Bhat, Kishore G.; and Bogar, Chetana (2019) "Cytotoxic activity of endophytes isolated from Allamanda blanchetii A. DC," Indian Journal of Health Sciences and Biomedical Research KLEU: Vol. 12: Iss. 2, Article 4.
DOI: https://doi.org/10.4103/kleuhsj.kleuhsj_7_19
Available at: https://kleijhsbr.researchcommons.org/journal/vol12/iss2/4

Pages

112

Last Page

116

Copyright

© 2019 Indian Journal of Health Sciences and Biomedical Research KLEU | Published by Wolters Kluwer – Medknow