To assess candidal colonization and species diversity in the oral cavity of diabetic and nondiabetic denture wearers and correlation with the presence of candida on finger tips of the patients An

Authors

Navjot Kaur Boparai, Departments of Prosthodontics and Crown and Bridge, Viswanath Katti Institute of Dental Sciences, Belgaum, Karnataka, India
Ulhas N. Amasi, Departments of Prosthodontics and Crown and Bridge, Viswanath Katti Institute of Dental Sciences, Belgaum, Karnataka, India
Raghunath Patil, Departments of Prosthodontics and Crown and Bridge, Viswanath Katti Institute of Dental Sciences, Belgaum, Karnataka, India
Sheetal U. Harakuni, Department of Microbiology, J.N. Medical College, KLE University′s, Belgaum, Karnataka, Indi

Abstract

Human oral cavity in health is normally colonized by different microbiological flora predominantly with

Candida

species. Wearing a removable denture and an immunologically deranged health is known to increase oral candidal colonization. The purpose of this study was to assess candidal colonization and species diversity in the oral cavity of diabetic and nondiabetic denture wearers and correlation with the presence of

Candida

on finger tips of the patients. 120 subjects in the age group of 40-70 years were included in the study. Three groups were formed, Group I: Diabetic, denture wearer, Group II: Nondiabetic denture wearer, Group III: Nondiabetic, nondenture wearer subjects. The oral samples from all the subjects were collected with oral rinse technique and were then inoculated on to the CHROMagar. For fingertip sampling, subjects placed their hands on sabouraud dextrose plate for 1 min and candidal growth was assessed and species were isolated using auxanometric plate method of sugar assimilation test. The mean number of colony forming unit (CFU) of

Candida

in Group I, Group II and Group III was 892.50, 590.00 and 152.50, respectively. Most commonly isolated species in diabetic denture wearer group were

Candida

albicans,

Candida

tropicalis

,

Candida

glabrata

,

Candida

krusei

,

Candida

kefyr

,

Candida

gulliermondi

and

Candida

parapsilosis.

The mean number of colonies for fingertip contamination was more for diabetic denture wearers (1.50) as compared to nondiabetic denture wearers (1.00). In conclusion, candidal carriage (based on the CFU) was more in diabetic denture wearer patients than the nondiabetic denture wearers.

C.

krusei

and

C.

kefyr

were the two species of

Candida

found only in diabetic subjects. Furthermore, there was a correlation in the fingertip contamination and oral candidal colonization.

Recommended Citation

Boparai, Navjot Kaur; Amasi, Ulhas N.; Patil, Raghunath; and Harakuni, Sheetal U. (2014) "To assess candidal colonization and species diversity in the oral cavity of diabetic and nondiabetic denture wearers and correlation with the presence of candida on finger tips of the patients An," Indian Journal of Health Sciences and Biomedical Research KLEU: Vol. 7: Iss. 1, Article 10.
DOI: https://doi.org/10.4103/2349-5006.135041
Available at: https://kleijhsbr.researchcommons.org/journal/vol7/iss1/10

Pages

45

Last Page

51

Copyright

© 2014 Indian Journal of Health Sciences and Biomedical Research KLEU | Published by Wolters Kluwer – Medknow

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